Universal Immunoprobe for (Per)Chlorate-Reducing Bacteria

Quantitative detection of perchlorate-reducing bacteria by real-time PCR targeting the perchlorate reductase gene.

A quantitative real-time PCR assay targeting the pcrA gene, encoding the catalytic subunit of perchlorate reductase, detected pcrA genes from perchlorate-reducing bacteria in three different genera and from soil microbial communities. Partial pcrA sequences indicated differences in the composition of perchlorate-reducing bacterial communities following exposure to different electron donors.

Perchlorate Reducing Bacteria: Evaluating the Potential for Growth Utilizing Nutrient Sources Identified on Mars

Introduction: Perchlorate reducing bacteria may serve as model organisms for potential life forms that could exist on Mars. Due to the high reduction potential of perchlorate it can be utilized in microbial metabolism as an electron acceptor [1]. Perchlorate was discovered to be present in martain surface materials (~0.5% wt.) using the Wet Chemistry Laboratory and the Thermal and Evolved Gas A...

Growth and Survival of Perchlorate-reducing Bacteria in Media Containing Elevated Perchlorate Concentrations and Uv-c Conditions

Introduction: The identification of perchlorate (ClO4) on Mars [1,2] has led to the possibility that complete redox couples are available for microbial metabolism in contemporary surface environments [3]. Perchlorate-reducing bacteria (PRB) utilize ClO4 and chlorate (ClO3) as terminal electron acceptors due to the high reduction potential [3]. Additionally, ClO4 salts have been suggested as a p...

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Kinetics of a chlorate-accumulating, perchlorate-reducing bacterium.

Kinetics parameters for perchlorate and chlorate reduction were determined for Dechlorosoma sp. HCAP-C, also known as Dechlorosoma sp. PCC, a novel perchlorate-reducing bacterium (PCRB) that accumulates significant amounts of chlorate during perchlorate reduction. This is the first report of such behavior, and we hypothesized the perchlorate reduction kinetics would be markedly different from o...